Your email address will not be published. Save my name, email, and website in this browser for the next time I comment. Sign me up for the newsletter! Share the Knowledge with your one Best Friend. Contents hide. About book: Name : Biochemistry. Edition: 8th. Publication: W. Pages: Content. About Authors:. Water 2. Amino Acids, Peptides, and Proteins 3. The Three-Dimensional Structure of Proteins 4. Protein Function 5.
Enzymes 6. Carbohydrates and Glycobiology 7. Nucleotides and Nucleic Acids 8. Features of Lehninger Principles of Biochemistry 7th Edition PDF: Following are the few important features of this book are given below; Provides a highly visual, reader-friendly approach to the challenging area of biochemistry.
Integrates the clinical perspective throughout the text, giving context and meaning to biochemistry. Frames every chapter with helpful synopses and summaries, and ends each chapter with review questions that reinforce major themes. Since we started with 10 mmol of the compound and an equal amount of HCl, then The remaining Therefore, the initial solution must have contained 1. Again using the Henderson- Hasselbalch equation, we can calculate pK A biochemist makes up mL of a 0.
She then adds 40 mL of 0. Answer The initial pH of 5. Initially, the pH was 5. Thus, in the initial solution, the ratio of [HisH ] to [His] is 4 to 1; 4 out of 5 of the imidazole groups were initially protonated. The initial solution contains The amount of HCl added was 4. The other 2. From these ratios of acid and base after the titration, we can calculate the final pH: pH 1.
She adds 6. What was the pH of the original solution? Answer First calculate the ratio of acid to conjugate base in the final solution: pH. The initial amount of the compound is 10 mmol mL 0. So after HCl addi- tion, 8. The amount of HCl added was 6. Now we can calculate the pH of the initial solution: pH. Phosphoric acid H3PO4 , a triprotic acid, has 3 pKa values: 2. Hint: Only one of the pKa values is relevant here. Preparation of Standard Buffer for Calibration of a pH Meter The glass electrode used in com- mercial pH meters gives an electrical response proportional to the concentration of hydrogen ion.
To convert these responses to a pH reading, the electrode must be calibrated against standard solutions of known H concentration. See Problem 22 for the pKa values of phosphoric acid. Answer In solution, the two salts ionize as indicated below. Therefore, to make 1. Briefly justify your answer.
Answer Acetic acid; its pKa is closest to the desired pH. Working with Buffers A buffer contains 0. Thus, the pH is 4. The added acid will convert some of the salt form to the acid form. Thus, the final pH is pH. Read the overview below and download using links given at the end of the post. With this edition, students will encounter new information emerging from high throughput DNA sequencing, x-ray crystallography, and the manipulation of genes and gene expression, and other techniques.
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